Part:BBa_K1090000:Design

AHL signal sender with RFP reporter under lac promoter control

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 863
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1450
  Illegal AgeI site found at 1562
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

This is primarily meant to be an AHL signal sender, but RFP is also used as a reporter gene to confirm expression from the lac promoter.

Source

E. coli K-12 MG1655, V. fischeri, and Genbank accession AF506027